A Plasmodium falciparum screening assay for anti-gametocyte drugs based on parasite lactate dehydrogenase detection

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A Plasmodium falciparum screening assay for anti-gametocyte drugs based on parasite lactate dehydrogenase detection.

OBJECTIVES Plasmodium gametocytes, responsible for malaria parasite transmission from humans to mosquitoes, represent a crucial target for new antimalarial drugs to achieve malaria elimination/eradication. We developed a novel colorimetric screening method for anti-gametocyte compounds based on the parasite lactate dehydrogenase (pLDH) assay, already standardized for asexual stages, to measure ...

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A Comparative in vitro Study of the Effect of Eosin B on Asexual Blood Stages and Gametocyte of Plasmodiun falciparum

Background and Objective: Malaria is one of the most life-threatening infectious diseases worldwide. Transmission of the parasite from human to vector mosquitoes is carried out by the gametocyte of the Plasmodium parasite, while these cells are not involved in the symptoms of the disease. The control of the human to mosquito  transmission stage of the parasite life cycle by antigametocyte drugs...

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A Flow Cytometry-Based Quantitative Drug Sensitivity Assay for All Plasmodium falciparum Gametocyte Stages

BACKGROUND Malaria elimination/eradication campaigns emphasize interruption of parasite transmission as a priority strategy. Screening for new drugs and vaccines against gametocytes is therefore urgently needed. However, current methods for sexual stage drug assays, usually performed by counting or via fluorescent markers are either laborious or restricted to a certain stage. Here we describe t...

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Glucose-6-phosphate dehydrogenase of malaria parasite Plasmodium falciparum.

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ژورنال

عنوان ژورنال: Journal of Antimicrobial Chemotherapy

سال: 2013

ISSN: 1460-2091,0305-7453

DOI: 10.1093/jac/dkt165